| | |
Curriculum Vitae
| Date of birth |
19/07/1978 |
| Country |
Italy |
| Job title |
Laboratory and student assistant |
| Previous job |
January 2004 – September 2004: Employed by University of Lausanne, Switzerland. My collaboration is based upon the continuation of the project I worked on in Zurich.
May 2003 - December 2003: Contracted as laboratory assistant by EAWAG (Swiss Federal Institute for Environmental Science and Technology) - Zurich, Switzerland.
Collaboration within the European research project BIOCARTE for the construction of bacterial biosensors based on à la carte regulators, in particular:
Mutagenesis and selection of HbpR variants with novel effector specificities
Engineering a site-specific chromosomal recombination system to assemble each of the novel regulators plus promoter and reporter gene within a predetermined site of the P.putida chromosome
Used techniques:
General molecular cloning techniques, DNA sequencing, random and site-directed mutagenesis, SDS-page, western blotting, protein purification, GMSA (gel mobility shift assay), standard microbiological methods, fluorimetry and fluorescent-assited cell sorting (FACS).
February 2003: University of Verona – Faculty of Natural-Physics-Mathematics Science - Verona, Italy. Degree in Agro-Industrial Biotechnology; final mark 109/110, in regular course of studies.
Discussion of the experimental thesis titled: “Phenotypic and phylogenetic characterisation of Burkholderia sp. strain DBT1, an interesting bacterium for bioremediation protocols”
February 2002 - October 2002: Master thesis at Helsinki University - Finland. College of Agriculture and Forestry. Department of applied chemistry and microbiology, Microbiology division.
Used techniques:
Phylogenetic analysis, primers and probe design for the characterisation of 16S rRNA, Real-Time PCR, standard microbiological methods, Fatty acid analysis, API 20 E – API 20 NE, BIOLOG GN2 Microplates, genomic DNA extraction, soil-DNA isolation, purification, PCR-amplification and gene cloning.
|
| Scholarship |
European Community |
| Language |
English: Fluent
French: Basic
German: No
Spanish: No
Other: italian: Mother tongue |
Informatic
Knowledge |
Excellent knowledge of Microsoft Office (Word, Excel, PowerPoint). Daily use of Internet Explorer; good knowledge of Adobe Photoshop, Adobe Illustrator, Adobe Acrobat Professional, Latex 2. Sequence analysis packages: Phylip, Clustal, FASTA, BLAST, DNASTAR (EditSeq, SeqMan, MegAlign), ARB. Knowledge on protein docking program GRAMM and on Swiss-Pdb Viewer. |
|
|
|
