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20 Messaggi |
 Inserito il - 12 aprile 2006 : 13:06:38
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Ciao a tutti, chiunque fosse interessato a fare un PhD con borsa Marie Curie legga questo annuncio. Buona fortuna.
Chiunqua volesse informazioni non esiti a contattarmi, vi ridirigo direttamente o al mio capo al mio collega che dovrebbe essere il supervisor.
Allegato:  Announcement recruitment (MYCOREMED).pdf
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Tat
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Città: Milano
33 Messaggi |
 Inserito il - 12 aprile 2006 : 17:13:59
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Ma che tipo di borsa è e soprattutto dove viene assegnata in che istituto?
Grazie  |
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20 Messaggi |
Inserito il - 12 aprile 2006 : 17:43:32
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| Puoi applicare ovunque nelle sedi descritte dal bando, hai letto l'allegato? La borsa é Marie Curie quindi il massimo per un dottorato in Europa. L'università dove sono io é la cattolica di Louvain la Neuve Belgio. Ci sono anche altre università. Scegli l'argomento che ti piace di più ed applica. Se ti interessa contattami in privato |
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20 Messaggi |
Inserito il - 12 aprile 2006 : 17:47:11
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cavolo ho provato ad aprire l'allegato e non si apre.
mandami tua mail in privato che ti invio l'annuncio.
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20 Messaggi |
Inserito il - 12 aprile 2006 : 18:00:19
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Allora questo é un word...a chi fosse interessato: mandatemi la vostra e-mail che vi mando il documento in pdf. Ciao
Contract Number
Project Title MYCOREMED - Role of arbuscular mycorrhizal fungi on the accumulation of radiocaesium by plants
Job Title PhD Positions
Job Description Soils contaminated with Cs pose a long-term radiation hazard to human health. Indeed, the chemical similarity of Cs and K constitutes the major threat to the contamination of the above-ground vegetation and incorporation in the food-chain as root uptake mechanisms appear closely related for these elements. Rhizospheric processes involving soil micro-organisms are known to influence radiocaesium root uptake. Among these, the obligate arbuscular mycorrhizal (AM) fungal symbionts are expected to play a key role due to their intimate position at soil/root interface. Recently, the role of AM fungi in Cs transport to the roots has been evidenced. Furthermore, it has been demonstrated that AM fungi can influence Cs root to shoot translocation by affecting Cs root transport. A recent hypothesis attributed to abscisic acid a primary role in restricting this transport. With this in view, the objective of the present proposal is to elucidate the mechanisms involved in the transport of Cs by AM fungi to the root and translocation from roots to shoot, leading to the evaluation of the radioecological significance of AM fungi on the accumulation of Cs in plants and to propose implementations of present bioremediation strategies.To achieve this goal five major fields of training, i.e. microbiology, molecular biology, biophysics, plant physiology and radioecology, will be considered.Four three-years PhD positions are available within this project :
PhD 1 : Determination of the mechanisms of Cs transport by AM fungi and effect of ABA on Cs transfer This research will be carried out at the UNIVERSITE CATHOLIQUE DE LOUVAIN (UCL) established in Belgium (contact person Stéphane Declerck – declerck@mbla.ucl.ac.be). The role of AM fungi in Cs transport has only been recently demonstrated thanks to the development of different innovative in vitro culture systems, using excised roots (Declerck et al., 2003; Dupré de Boulois et al. 2005) or autotrophic plants (Dupré de Boulois et al., in press). With these systems, uptake, translocation and transfer of Cs from AM fungi to their host root have been observed while its translocation from root cells to shoots cells was shown to be extremely low. This later result suggested that AM fungi could influence root Cs transport by reducing its loading into the xylem and therefore impair root to shoot translocation.The goal of this research will be to identify the mechanisms of Cs transport by AM fungi by following 3 steps. The first one will consist on the determination of the mode of uptake of Cs+ (i.e. passive or active) by the extraradical hyphae of AM fungi, and to identify, if applicable, the type of transporters involved in this uptake and their affinity. The second step will aim at defining the mode of translocation of Cs+ along mycorrhizal hyphae. The third one will consist on the determination of the rate of Cs+ transfer at the fungal-plant interface, the determination of the mode of transport, and to identify, if applicable, the type of transporters involved in the efflux of Cs+ into the interfacial space between intraradical fungal structures (i.e. arbuscules) and root cells and influx into the root cells. In this later step, the influence of ABA in the transfer of Cs to root cells will also be investigated. The Arbuscular Mycorrhiza – Plant (AM-P) in vitro culture system will be used in this task. This system is the most advanced cultivation system for studies on element transport under strict in vitro conditions since it associates photosynthetic active plants with an AM fungal strain (Dupré de Boulois et al., in press). This cultivation system was long awaited by the scientific community as it can advantageously replace the root organ culture (ROC) system for studies involving a “source-sink” relationship. In addition, this upgraded in vitro culture system is, as for the ROC system, perfectly adapted for plant and fungal gene expression (Kosuta et al. 2003), physiological and biophysical (Olsson et al., 2002; Pfeffer et al., 2004) and radioecological studies (Rufyikiri et al., 2002, 2003, 2004; Declerck et al., 2003; Dupré de Boulois et al., 2005).
PhD 2 : Identification and expression of K/Cs transporters in mycorrhizal and non-mycorrhizal roots This research will be carried out at the University of Warwick - Horticulture Research International (W – HRI) established in Great Britain (contact person Philip J. White - philip-j.white@warwick.ac.uk).This task consists on the molecular identification of the transporters involved in Cs transport in roots cells (i.e. influx and efflux) and the determination of the expression of these transporters in mycorrhizal plants versus non-mycorrhizal plants. This will be done by performing homology screening of corresponding sequences known in other plant species and by semi- and quantitative determination of gene expression, respectively. In addition, the role of ABA on the expression of these transporters will also be investigated.The W-HRI team have experience in monitoring the changes in gene expression in response to mineral stresses, including N, P and K deficiencies and Cs toxicity (Hammond et al. 2003; Hampton et al. 2004). Gene expression in plant tissues will be monitored using commercial and/or customised microarrays. The hardware and software tools for the transcriptional analysis of plant species for which microarray technology (or sequence data) is unavailable have been developed by W-HRI in collaboration with Nottingham Arabidopsis Stock Centre (NASC, UK; Okyere et al. 2005). Thus, the effects of AM fungi on global plant gene expression will be catalogued. The results will be verified using quantitative PCR, and the function of specific genes will be studied using conventional molecular biological techniques, including RNAi trangenesis. Real time PCR will also be used for expression analysis. The method is very sensitive (needs very few material) and much more precise than Northern blots.
UW (host of PhD 3) was the first institution which published real time PCR expression analyses from plant material (Szyroki et al., 2001) and uses the method routinely (e.g. Reintanz et al., 2002; Langer et al. 2002) and is now mastered by W-HRI.PhD 3 : Identification of ABA regulatory pathways involved in Cs root transport This research will be carried out at the Julius-v.-Sachs-Institut für Biowissenschaften Molekulare Pflanzenphysiologie und Biophysik of the University of Würzburg established in Germany (contact person Rainer Hedrich - hedrich@botanik.uni-wuerzburg.de). The determination of the influence of AM fungi on ABA root levels will be conducted by analysing ABA levels during AM fungus – plant interaction. Thereafter, investigation of the function and role of ABA regulatory pathways for K/Cs transporter regulation, H+-ATPase activity at the AM fungus-plant interface and cytosolic pH alteration caused by ABA will be investigated.The physiological and biophysical studies performed in UW will use the most advanced existing techniques. The double electrode voltage clamp (DEVC) technique is a method for characterisation of single electrogenic transport proteins in the heterologous expression system of Xenopus laevis oocytes. It has widely been used to characterize cloned electrogenic transporters in vitro. Examination of structure-function relation by expressing transporters with altered (mutated) amino acids and co-expression studies have been performed by UW (Hoth et al. 1997; Hoth et al., 2001; Geiger et al., 2002; Klepek et al., 2005; Carpaneto et al, in press). DEVC will reveal K/Cs transporter properties, which might be compared to in vivo data. Patch clamp technique is an electrophysiological method for studying plant ion channels on single protoplasts. The method comprises different applications to assess ion channel function. The four common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. In contrast to the DEVC, where one channel type without the electrical background of the in vivo plant system is studied, the patch clamp technique analyses ion channels in their natural environment on the level of single protoplasts. Prof. Hedrich (UW) was the first who applied the technique on plant cells (Schroeder et al., 1984; Hedrich and Neher, 1987). The technique has successfully been used to characterise the electrical properties of root protoplasts (Paganetto et al., 2001; Ivashikina et al., 2001; Reintanz et al., 2002). Single cell electrode technique (Impalement method) uses sharp microelectrodes to impale living cells of intact plants. This technique, developed in the group of Prof. Hedrich (UW), gives access to the electrical properties of living cells in their natural tissue environment (Roelfsema 2001a and b; Roelfsema et al., 2002; Roelfsema et al. 2004; Levchenko et al., 2005). Using multi-barreled electrodes changes in the free running membrane potential and in whole cell currents can be simultaneously monitored. In addition, ion activities or signal transduction pathways can be studied by injection of ion-sensitive dyes or signal molecules directly into the cytoplasm of the cells. The method can, for instance, be used to observe ion uptake via root hairs. Single cell techniques: AM fungi have several consequences on mineral uptake and the nutritional status of the plant. In order to differentiate between expression pattern of mycorrhizal and root cortex cells, these methods are required as they give access to single cells. One possible approach is to isolate protoplasts of the desired cell type that are somehow labelled (Ivashikina et al., 2003). To circumvent the disadvantage of altered expression patterns by the enzymatic treatment, the laser micro dissection (LMD) provides a powerful tool to examine the border between fungus and plant (Kerk et al., 2003; Ivashikina et al., 2003). Since the amount of RNA obtained by the above-mentioned approaches is very limited, UW currently develops a linear RNA amplification protocol without fragmentation of the target material.
PhD 4 : Radioecological significance of the influence of AM fungi on Cs plant accumulationThis research will be carried out at the Institute of Botany of the Jagiellonian University established in Poland (contact person Katarzyna Turnau - ubturnau@kinga.cyf-kr.edu.pl).In order to identify the extent of AM fungi influence on Cs acquisition and accumulation by plants, different symbiotic AM associations (AM fungi and plants) and scenarios such as various [K+]ext, pHext levels or [Pi]ext levels will be tested. In addition, the distribution of Cs as a function of the AM association development will be measured in order to know if the partition of Cs between root and shoot may evolve during the symbiotic interaction (i.e. extend of root colonisation, seasonal variations). Finally, the radioecological significance of the influence of AM fungi on Cs plant accumulation will be determined and early assessment of the technological feasibility and advantages (e.g. cost-benefit analysis and environmental impact assessment) that AM fungi can offer to phytoremediation strategies will be performedRadioecological tools will be used in this task. They will especially involve micro-analytical tools related to optical or electron microscopy. The localisation of elements at the cellular and subcellular levels using one of the micro-analytical methods, such as EDS, gives important data on element distribution, and thus on the role of elements in physiological processes, interactions between elements and reasons for their deficiency or toxicity. It also provides the link between physiological and anatomical studies, which is especially important when studying responses of organisms to environmental stress such as the presence of naturally occurring heavy metals or metals introduced by pollution (Turnau and Kottke, 2005).The four PhD students will have the opportunity to spend periods in all the institutions involved in the project to learn techniques and conduct complementary experiments to answer the questions of MYCOREMED.
The PhD studentships will be available from July 2006 for a period of three years.Criteria for recruitment of young fellows will be· High grade academic curriculum vitae in most appropriate discipline· Interest and motivation in conducting the research activity and accomplishment of PhD · Interest in mobility· Curiosity · Agreeable personality and integration capacity within teamsThe young fellows should send their Europass CV, Europass Language Passeport and motivation letter to the contact person of the PhD subject of interest and to Prof. S. Declerck (address below) as coordinator of the proposal. The EUROPASS CV will be used as the backbone for recruitment as it enables skills and qualifications to be clearly visible and identifiable, and to have a same frame for all candidates. To this document, the EUROPASS LANGUAGE PASSEPORT will be required and if applicable, the candidates will be invited to join EUROPASS MOBILITY and DIPLOMA SUPPLEMENT documents.
These documents can be found on the EUROPASS web portal : http://europass.cedefop.eu.int/htm/ or at the candidates’ national ENIC/NARIC centres. Applications should be in English.Following early recruitment, the selected candidates will be examined by an interview committee.Interviews will be performed in English. Concerning equitable balance between men and women, each partner will integrate the gender dimension in FP6 (http://europa.eu.int/comm/employment_social/equ_opp/index_en.htm) to ensure equal employment opportunities for both sexes. All partners of the consortium are equal opportunity employers in terms of gender, race and disability. Therefore, interview committees for job applicants are balanced between males and females.
Contract Type Temporary
Position Early Stage researcher (max. 4 years experience)
Number of Positions 4
Deadline 01/06/2006
Start Date 01/08/2005
Durationin months 36
Yearly living allowance Approx. 31 000 euros
Contact Prof. S. DeclerckUniversity catholique de Louvain declerck@mbla.ucl.ac.be http://www.mbla.ucl.ac.be/cesamm
Disciplines Environmental science
Town Louvain-la-Neuve – Warwick – Würzburg - Kraków
Country Belgium – Great Britain – Germany - Poland
How to apply By e-mail
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AleXo
Moderatore
Prov.: Estero
Città: San Francisco, California
1550 Messaggi |
Inserito il - 12 aprile 2006 : 19:53:16
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le azioni Marie Curie sono davvero una delle migliori opportunità che offre l'europa.
Peccato che il sito sia veramente pessimo
Vi riporto qui altre due borse Marie Curie disponibili:
http://www.biomalpar.org/EMGS_advert_homepage.html
http://www.ucd.ie/conway/ed_postgraduate2.html
Il primo offre una serie di progetti, anche molto diversi fra loro, sulla Malaria. I laboratori sono dislocati in tutta europa e la struttura del programma ricalca quello dei phd EMBL
Purtroppo sono fuori per questioni di tempo
Il secondo invece è in NeuroImmunologia Molecolare, presso l'university College of Dublin.
ricordate che la scandenza per entrambi è molto prossima!!
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AleXo
Moderatore
Prov.: Estero
Città: San Francisco, California
1550 Messaggi |
Inserito il - 25 aprile 2006 : 00:25:39
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un altro: http://www.uku.fi/nucsys/ 
nel network di questo phd marie curie c'è anche una biotech Italiana: la BioXell, spin-off Roche, nel science park del San Raff a Milano. |
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AleXo
Moderatore
Prov.: Estero
Città: San Francisco, California
1550 Messaggi |
Inserito il - 06 ottobre 2006 : 12:16:10
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PhD position at Malmö University.
The candidate should be European, but not Swedish or German. For Swedish or German citizens, follow the link and you will find PhD positions att other countries.
http://europa.eu.int/eracareers/index_en.cfm?l1=1&l2=1&l3=1&IdJob=14450561&CFID=869056&CFTOKEN=
Topic11: Cell-to-bio-mimetic interface interactions
Position WP11_1:
Place of work: Malmö University (PhD in biomedical technology), Sweden (24 month) and GKSS Research Center Geesthacht, Germany (12 month)
Contact person: Prof. Thomas Arnebrant (Thomas.Arnebrant@hs.mah.se) or
PD Dr. Regine Willumeit (regine.willumeit@gkss.de)
Project description: The aim of this PhD project is to monitor and control the cellular interaction with bio-mimetic interfaces (i) by characterising focal adhesion complexes of adherent eukaryotic cells by electrical impedance, ellipsometric, AFM and QCM measurements, (ii) by changing bio-mimetic bio-interfaces to control characteristics of focal adhesion complexes and (iii) by correlating characteristics of focal adhesions with metastatic potential of cancer cells and tissue-implant interactions.
Requirements: The candidate should have excellent basic education in chemistry or biochemistry. Knowledge in cell biology is of high advantage.
Tautgirdas Ruzgas, PhD, Docent
Biomedical Laboratory Science
Faculty of Health and Society
UMAS Entrance 49
Malmö University
Södra Förstadsgatan 101
SE-20506 Malmö
Sweden
http://www.mah.se/biofilms
NdAleXo: ho ricevuto quest'offerta via mail... Malmö è carina ed è a mezz'ora da Copenhagen (che è stupenda)!
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Possibilitˆ di PhD-Marie Curie-
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