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Messaggio inserito da Smichel

ciao a tutti,

dire sequenziamento 454 e Pirosequenziamento è la stessa cosa?


Grazie a tutti.

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Citazione:

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Technology

454 Sequencing is a large-scale parallel pyrosequencing system capable of sequencing roughly 400-600 megabases of DNA per 10-hour run on the Genome Sequencer FLX with GS FLX Titanium series reagents. The technology is known for its unbiased sample preparation and long, highly accurate sequence reads (400-500 base pairs in length), including paired reads.[citation needed] Software analysis tools, including an assembler, mapper and amplicon variant analyzer, are included with the system.

The system relies on fixing nebulized and adapter-ligated DNA fragments to small DNA-capture beads in a water-in-oil emulsion. The DNA fixed to these beads is then amplified by PCR. Each DNA-bound bead is placed into a ~29 #956;m well on a PicoTiterPlate, a fiber optic chip. A mix of enzymes such as DNA polymerase, ATP sulfurylase, and luciferase are also packed into the well. The PicoTiterPlate is then placed into the GS FLX System for sequencing.
(da Wikipedia)

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