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chick80
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Città: Edinburgh
11491 Messaggi |
 Inserito il - 06 novembre 2006 : 21:57:18
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Da: C. Nälsén, M. Öhrvall, A. Kamal#8208;eldin, B. Vessby
Plasma antioxidant capacity among middle-aged men: The contribution of uric acid
Measurement of plasma antioxidant capacity (AOC)
AOC was measured using an enhanced chemiluminescence assay [3]. This technique is based on the measurement of light emission from a chemiluminescent substrate such as luminol when it is oxidized in a reaction catalysed by horseradish peroxidase. Because light emission is suppressed by radical scavenging antioxidants, the duration of light suppression is related to the amount of antioxidant present. Light emission was measured with a luminometer (1251 Luminometer; BioOrbit, Turku, Finland), and the sensitivity was set at 4. To initiate a chemiluminescent reaction, 890 µL plasma (diluted in millipore water, 1:445, v/v) was dispensed into a cuvette in the luminometer. Subsequently, 10 µL horseradish peroxidase solution (diluted 1:200, v/v) (Amersham International, Amersham, UK) and 100 µL signal reagent consisted of luminol, enhancer and oxidant (Amersham International) were added, and light emission was measured for 4 min. The AOC of samples was quantified by comparing the duration of light suppression to that induced by the tocopherol-analogue, Trolox (Aldrich Chemie, Steinheim, Germany). Thereafter, AOC was quantified relatively and expressed as µmol Trolox equivalents per litre of plasma. Fresh horseradish peroxidase- and Trolox solutions were prepared daily.
L'articolo a cui fanno riferimento è: Enhanced chemiluminescent assay for antioxidant capacity in biological fluids |
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capacità antiossidante del plasma
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