Ragazzi ho da fare un problema di genetica ma non ci capisco gran che! il problema dice :
A human autosomal recessive disease may be investigated using the PCR followed by restriction enzyme analysis of the reaction products with the enzyme HindIII. The gene responsible for the disease has two alleles (A1 and A2). In both cases the PCR yields a product of 900 base pairs but HindIII only cleaves the product of one of the two alleles (A2) generating fragments of 700 and 200 base pairs. A couple with an affected child and expecting twins has been genetically tested for this disease and the sizes of the DNA fragments obtained after PCR and restriction enzyme analysis are shown in the Table below:
Fragment sizes (base pairs)
Father 900, 700, 200
Mother 900, 700, 200
Affected child 700, 200
Foetus A 700, 200
Foetus B 900, 700, 200
Using this information:
i) determine the genotype of the father, the mother, the affected child and the two foetuses
ii) identify the mutant allele
iii) discuss the genetic make-up of the twin foetuses and explain whether they are likely to be affected by the disease
SAPETE COME USCIRNE?? STO IMPAZZENDO! SONO UN VIROLOGO E DI GENETICA NON CI CAPISCO GRAN CHE ! :(
Genome mapping
Inserito il - 30 ottobre 2012 : 15:43:38
