Bioinformatica e Web 2.0

Inside Bioinfo

2 dicembre 2023 - 17:49

Decodificami! La Scienza si fa Arte (e Moda)

Can drinking excessive water help pass a drug test?

Drug tests are crucial in various fields, from employment screenings to sports competitions, ensuring safety and fair play. However, myths persist about methods to pass these tests, with one common belief revolving around excessive water consumption as a solution click

Understanding Drug Tests
There are different types of drug tests, including urine, blood, hair, and saliva tests. Each has its own detection window, influencing how long substances remain detectable in the body.

Role of Water in the Body
Water is essential for bodily functions, aiding in digestion, circulation, and toxin removal. The body regulates water balance through various mechanisms, ensuring proper hydration levels.

Can Drinking Water Help Pass a Drug Test?
The idea behind excessive water intake is to dilute urine, potentially reducing the concentration of detectable substances. However, this method poses risks and may not guarantee passing a drug test.

Other Methods to Pass a Drug Test
Natural detoxification processes, such as increasing physical activity and a healthy diet, might aid in eliminating toxins. Additionally, some commercial products claim to aid in detoxification before a drug test.

Health Risks of Excessive Water Consumption
Consuming an excessive amount of water in a short period can lead to water intoxication, causing electrolyte imbalances that may result in serious health complications.

While drinking water is essential for overall health, relying solely on excessive consumption to pass a drug test is ineffective and risky. Exploring safer alternatives and understanding the limitations of water dilution is crucial.

24 marzo 2009 - 19:59

Disegnare velocemente Primer con CLC…

Differentiating Between Natural and Synthetic Nootropics

In the world of nootropics, there are two main categories: natural compounds derived from plants and synthetic drugs created in laboratories. Understanding the differences between these options can help individuals make informed choices here

Natural Nootropics: Traditional Use without Extensive Research

Natural nootropics are often derived from herbs or other plant-based sources. These ingredients have a long history of traditional use in various cultures for their cognitive-enhancing properties. However, it’s important to note that while they may have been used for centuries, extensive scientific research on their effectiveness is often lacking.


Long history of traditional use

Generally considered safe with minimal side effects

Availability as dietary supplements without prescription


Limited scientific evidence supporting their efficacy

Varying potency and quality among different brands or sources

Lack of standardized dosages and formulations

Examples of herbal nootropics include ginkgo biloba, bacopa monnieri, and rhodiola rosea. These natural compounds are believed to improve memory, concentration, and overall brain function. While anecdotal evidence suggests positive effects, more research is needed to validate these claims.

Synthetic Nootropics: Designed for Cognitive Enhancement

On the other hand, synthetic nootropics are specifically designed in laboratories with the goal of enhancing cognitive function. These compounds undergo rigorous testing to ensure safety and efficacy. They are typically created to target specific areas of cognition such as memory retention or mental focus.


Extensive scientific research supporting their effectiveness

Precise control over dosage and formulation

Potential for targeted cognitive enhancement


Increased risk of side effects compared to natural options

May require a prescription or medical supervision

Limited availability due to regulatory restrictions

Synthetic nootropics include compounds like modafinil, piracetam, and aniracetam. These drugs have been studied extensively and are known to enhance cognitive abilities in specific ways. For example, modafinil is often prescribed to treat sleep disorders and has gained popularity as a cognitive enhancer due to its ability to promote wakefulness and increase focus.

It’s important to note that while synthetic nootropics may offer more targeted effects and scientific evidence, they also come with a higher risk of side effects compared to natural options. Therefore, it’s crucial for individuals considering synthetic compounds to consult with a healthcare professional before use.

Understanding the Mechanism of Weight Loss Drugs

Weight loss drugs can be a helpful tool for individuals looking to shed those extra pounds. But have you ever wondered how these medications actually work in your body? Let’s dive into the mechanisms behind different types of weight loss drugs and how they contribute to overall weight management more information

Different Types of Weight Loss Drugs

There are various types of weight loss drugs available on the market, each with its own unique mechanism of action. Some common categories include appetite suppressants, fat absorption inhibitors, and metabolism enhancers.

Appetite Suppressants

One popular mechanism used by weight loss drugs is appetite suppression. These medications work by targeting specific receptors in the brain that regulate hunger and satiety. By activating these receptors, appetite suppressants help reduce feelings of hunger and increase feelings of fullness, leading to decreased food intake.


Can help individuals control their portion sizes and reduce calorie intake.

May lead to more sustainable weight loss when combined with a healthy diet and exercise.


Potential side effects such as dry mouth, insomnia, or increased heart rate.

Not suitable for everyone, especially those with certain medical conditions or taking specific medications.

Fat Absorption Inhibitors

Another type of weight loss drug inhibits the absorption of dietary fats in the intestines. These medications work by blocking enzymes responsible for breaking down fats during digestion. As a result, less fat is absorbed into the bloodstream, reducing overall calorie intake.


Can be effective in reducing body weight when combined with a low-fat diet.

May also improve cholesterol levels.


Can cause gastrointestinal side effects like oily stools or diarrhea.

Not recommended for individuals with certain digestive disorders or vitamin deficiencies.

Metabolism Enhancers

Metabolism-enhancing weight loss drugs aim to increase the body’s energy expenditure by boosting metabolism. These medications work by targeting specific receptors or enzymes involved in metabolic processes, such as the activation of brown adipose tissue (BAT) or the inhibition of monoamine oxidase.

A seconda del tipo di PCR che si deve fare (Real Time Pcr o no, da genomico o da plasmide) si devono costruire primer più o meno “accurati”. Se si deve fare una Pcr da genomico ad esempio bisogna stare attenti che i primer non vadano anche ad annilare in altri punti del genoma, oltre a quello di interesse…

Nel mio caso faccio molte PCR da plasmidi. In questo caso le coppie di basi non sono molte, quindi è altamente improbabile che primer lunghi 15-20 nucleotidi vadano ad annilare in più punti.

In questo caso disegnare dei primer è molto semplice…Ma troppe volte vedo persone che disegnano su carta i primer, sia forward che reverse (perdendo tempo e rischiando di fare errori).

La cosa è molto più semplice con CLC Sequence Viewer:

  • (Ovviamente) Importare la sequenza del gene su cui fare la PCR in CLC Sequence Viewer
  • Selezionare lo spaziamento ogni 3 nucleotidi scegliendo il frame appropiato (Potete anche salvare questa opzione in modo da non selezionarla ogni volta).


  • Selezionare i 15-20 nucleotidi che formeranno il primer FW (tenendo conto del frame e che devono terminare con una C o G), poi tasto dx -> Open Selection in new view
  • Tasto dx sul primer appena creato -> Select Sequence, di nuovo tasto dx -> Edit. In questo modo potete modificare la sequenza, aggiungere codoni di start, enzimi di restrizione.
  • Tornando sulla sequenza del gene selezionate il segmento che andrà a formare il primer Reverse. In questo caso non c’è bisogno di tenere conto del frame. Fate in modo che cominci con una G o una C (l’inizio che selezionate diventerà il 3?, quindi rappresenta la fine del primer). Al solito, fate tasto dx -> Open Selection in new view. Poi modificate il primer a vostro piacere aggiungendo codone di STOP o siti di restrizione.
  • A questo punto nella toolbox “Reverse Complemente Sequence” e avrete il vostro primer Reverse pronto.


I primer possono essere copiati-incollati sul sito dove ordinate i primer (evitate errori di battitura), possono essere stampati o salvati in una cartella. Vi ricordo che se si deve fare una Pcr da genomico bisogna stare attenti che i primer non vadano anche ad annilare in altri punti del genoma, quindi questo metodo non va bene (in questo caso c’è il sistema descritto da Nico).

Spero però sia utile a qualcuno!!

Alla prossima…

Link: MyBioinformatica

Tags: bioinformatica, how-to
16 dicembre 2008 - 20:04

Editor di sequenze: CLC Sequence Viewer

Un editor di sequenze è un programma in grado di manipolare con facilità sequenze nucleotidiche e proteiche. Il primo dei programmi che ho intenzione di presentarvi è CLC Sequence Viewer che è arrivato alla versione 5.1.1

Il programma è creato dalla CLC bio, che permette il download e l’utilizzo gratis, indubbiamente presenta una grafica molto curata, ed una facilità d’uso impressionante, adatto anche a chi non sa bene usare il computer.

Understanding CBD and Its Effects on Sleep

Research suggests that CBD interacts with the body’s endocannabinoid system, which plays a crucial role in regulating various physiological processes, including sleep. By influencing receptors in the brain and nervous system, CBD may help alleviate symptoms of insomnia and promote a more restful night’s sleep.

How CBD Gummies Work for Sleep
CBD gummies are a convenient and tasty way to consume CBD. When you ingest a CBD gummy, it is absorbed into your bloodstream through the digestive system. From there, it interacts with receptors in the body to produce its effects, including promoting relaxation and reducing anxiety, both of which can contribute to better sleep.

Benefits of Using CBD Gummies for Sleep
Natural: CBD gummies are made from natural ingredients and are free from artificial additives.
Non-psychoactive: Unlike THC, CBD does not produce a psychoactive high, making it safe for daily use.
Convenient: CBD gummies are easy to take on the go and can be consumed discreetly.
Relaxing: CBD promotes relaxation, which can help ease anxiety and stress, leading to better sleep quality.
Customizable: CBD gummies come in various strengths and flavors, allowing users to find the perfect option for their needs.
Choosing the Right CBD Gummies for Better Sleep
When shopping for CBD gummies, it’s essential to choose a reputable brand that uses high-quality ingredients and follows strict manufacturing standards. Look for products that have been independently tested for potency and purity to ensure you’re getting a safe and effective product.

Potential Side Effects of CBD Gummies
While CBD is generally well-tolerated, some people may experience side effects such as dry mouth, dizziness, or changes in appetite. It’s essential to start with a low dose and gradually increase as needed to minimize the risk of adverse effects.

Dosage Recommendations for CBD Gummies
Dosage can vary depending on factors such as body weight, metabolism, and the severity of symptoms. It’s best to start with a low dose and gradually increase until you find the right amount for you. Consult with a healthcare professional for personalized guidance.

Tips for Incorporating CBD Gummies into Your Sleep Routine
Consistency: Take your CBD gummies at the same time each night to establish a routine.
Relaxation: Pair your CBD gummies with relaxing activities such as meditation or gentle stretching.
Limit Stimulants: Avoid caffeine and electronic devices before bedtime to promote better sleep.
Customer Reviews and Success Stories
Many people have reported positive experiences with CBD gummies for sleep. From reduced anxiety to improved sleep quality, the anecdotal evidence is promising. However, individual results may vary, and it’s essential to find what works best for you.

CBD gummies offer a natural and convenient option for promoting better sleep. By harnessing the relaxing properties of CBD, these tasty treats can help ease anxiety and stress, leading to a more restful night’s sleep. Whether you’re struggling with insomnia or simply looking to enhance your sleep routine, CBD gummies may be worth exploring.

Frequently Asked Questions About CBD Gummies for Sleep
What are CBD gummies?
CBD gummies are edible candies infused with CBD oil. They offer a convenient and tasty way to consume CBD and are available in various flavors and strengths.

Can CBD gummies help with sleep?
Many people report that CBD gummies help them relax and improve their sleep quality. CBD may interact with receptors in the body to promote relaxation and reduce anxiety, both of which can contribute to better sleep.

Are CBD gummies legal?
CBD gummies derived from hemp containing less than 0.3% THC are legal at the federal level in the United States. However, laws regarding CBD vary by state, so it’s essential to check your local regulations.
Questo programma presenta tante limitazioni rispetto agli altri programmi (a pagamento) della CLC bio.
Le operazioni possibili sono (e le limitazioni relative sono):

  • effettuare ricerche direttamente su NCBI, anche se a causa delle limitazioni non è possibile effettuare ricerche su altri database, bisogna comprare le altre versioni.
  • creare allineamenti multipli e relativi alberi, con vari algoritmi: Clustal, T-coffe, ecc…
  • varie operazioni con i nucleotidi: convertire Dna <-> Rna, vedere filamento complementare di una sequenza, tradurla in proteina, Cercare ORF, ecc…
  • Cercare enzimi di restrizione presenti in una sequenza ed elencarli in una lista.

Quando si effettua una ricerca su NCBI, o si importa una sequenza, CLC Sequence Viewer è in grado di leggere le annotazioni e visualizzarle graficamente, indicando ad esempio promotori, la sequenza codificante in un cDna, domini e così via…Una grossa limitazione che ho trovato è non poter modificare le annotazioni riguardanti le sequenze. Quindi non è possibile selezionare una serie di nucleotidi/amminoacidi e aggiungere un’annotazione ad esse (ad es. questo è un promotore, qui c’è un sito di fosforilazione, ecc). Questa forte limitazione (e il fatto che secondo me i programmi dovrebbero essere liberi) mi ha spinto a cercare un altro editor di testo.

Qui è presente l’elenco completo delle limitazioni, paragonate ai relativi software a pagamento.

Il programma è disponibile per Windows, Mac, Linux (sia file .rpm che .sh), ed è molto semplice da installare.

9 dicembre 2008 - 09:00

How to Get Rid of Fleas in Your House, in Your Yard, and More

Understanding Metformin and its Role in Weight Loss

Metformin is a widely used medication primarily prescribed for the management of type 2 diabetes. However, in recent years, it has gained attention for its potential role in aiding weight loss. This article aims to delve deeper into the understanding of Metformin and its efficacy in promoting weight loss.

Metformin and Weight Loss
Metformin’s association with weight loss stems from its impact on various metabolic processes within the body. While its primary function is to improve insulin sensitivity and regulate blood sugar levels, it also exhibits properties that can contribute to weight reduction.

Mechanism of Action
The precise mechanism through which Metformin induces weight loss is multifaceted. One of its primary actions involves decreasing the liver’s production of glucose while simultaneously enhancing insulin sensitivity in peripheral tissues. By reducing excess glucose production and improving insulin utilization, Metformin helps the body utilize glucose more efficiently, thereby promoting weight loss.

Effects on Insulin and Blood Sugar Levels
Individuals with insulin resistance often experience difficulty in losing weight due to impaired glucose metabolism. Metformin addresses this issue by enhancing insulin sensitivity, allowing cells to respond more effectively to insulin’s signals. Additionally, by lowering blood sugar levels, Metformin helps prevent the excessive release of insulin, which can contribute to fat storage.

Research Studies
Numerous research studies have investigated Metformin’s effects on weight loss, with many demonstrating promising results. These studies have highlighted Metformin’s ability to facilitate modest but significant weight reduction, particularly in individuals with insulin resistance or obesity-related conditions.

Dosage and Administration
When used for weight loss purposes, Metformin is typically prescribed at lower doses compared to its diabetes management dosages. Healthcare providers may recommend starting with a low dose and gradually titrating upwards to minimize potential side effects while maximizing efficacy.

Potential Side Effects
Like any medication, Metformin is not without side effects. Common side effects include gastrointestinal discomfort such as nausea, diarrhea, and abdominal cramping. However, these symptoms often subside over time or with dosage adjustments.

Who Can Benefit from Metformin for Weight Loss?
Metformin may be beneficial for individuals struggling with weight loss, particularly those with insulin resistance, prediabetes, or polycystic ovary syndrome (PCOS). It is essential to consult with a healthcare provider to determine if Metformin is suitable for individual needs.

Precautions and Considerations
Before initiating Metformin therapy for weight loss, it is crucial to consider various factors such as medical history, existing health conditions, and potential drug interactions. Pregnant or breastfeeding women and individuals with kidney or liver impairment should exercise caution when using Metformin.

Combination Therapy
Combining Metformin with other weight loss strategies such as diet modification and regular exercise can enhance its effectiveness. Lifestyle changes play a significant role in achieving sustainable weight loss outcomes.

Consultation with Healthcare Providers
Prior to starting Metformin for weight loss, individuals should consult with a healthcare provider to assess suitability, discuss potential risks and benefits, and establish an appropriate treatment plan. Regular monitoring and follow-up are essential to ensure safe and effective use of Metformin.

Real-life Success Stories
Many individuals have experienced success in achieving weight loss goals with the help of Metformin. These success stories underscore the potential benefits of incorporating Metformin into comprehensive weight management strategies.

Fleas are some of the most annoying pests to deal with. They’re small, jumpy, and multiply quickly. Pets can pick up fleas from being outside in nature, around other animals, or when humans track in the insects on our shoes or clothes.

Excessive itching and scratching is a telltale sign your pet may have fleas. You may even spot the little acrobats. On average, fleas are 2-4 millimeters long, making them visible to the naked eye.

If your family pet has fleas, it’s likely that your yard and house will become a breeding ground. Acting quickly is key to preventing spread.

A flea infestation can test your patience and require persistence. But you can eradicate the problem with a combination of cleaning methods, sprays, and pet-friendly topical medications among other options.

Do fleas bite people, too?

Yes! Fleas are after blood and can bite people or latch onto our clothes or shoes. However, they really do prefer animals. Pets are ideal hosts because thick fur provides plenty of shelter for fleas to latch onto skin and feed, or lay eggs.

If fleas do bite you during an infestation, it will likely be around your ankles, or in folds of skin. Flea bites can cause an allergic reaction in the form of hives.


According to the Centers for Disease Control, the life cycle of the fleaTrusted Source depends on a lot on environmental conditions. Fleas flourish in warm climates and usually (but not always) die in the winter season.

Flea eggs are small but can be seen if you’re looking closely. They are smooth and white or light in color. A single adult female flea can produce up to 2,000 eggs in her lifetime. These eggs may be laid in your pet’s fur, deep in the carpet, or in tall grass.

In ideal conditions, fleas will evolve from egg to adult within 2 to 3 weeks. Adult fleas can live up to 100 days.

Fleas are ready to feed within a day of hatching, and begin to suck blood within 10 seconds of landing on a host.

If the weather isn’t ideal and there isn’t a host to feed on, flea larvae may remain dormant for months while waiting for better conditions to develop. This is a key reason dealing with infestations can be so difficult.

How long does it take to get rid of fleas?

The length of time required to get rid of a flea infestation depends on your environment, and how long the fleas have been there.

Prompt cleaning and using topical flea medications for your pet may get rid of the majority of fleas within a day or two. However, it can take days to weeks for all the fleas present in an environment to die, even with the most conscientious approach.

Fleas lay a lot of eggs very quickly, and some fleas have developed resistance to medications and insecticides. If you have a large property or multiple pets with fleas, it may take longer to get rid of the problem. The key to handling fleas is persistence.

How to get rid of fleas in your home

If your pet is a walking carrier of mature fleas, your home can become the nursery.

Since the flea has multiple life stages (egg, larvae, cocoon, adult), when adult fleas are present, it is assumed all of these stages are also present throughout your house. This means that you have to tackle the problem from all angles in order to truly eradicate the infestation. Learn more detailed information from this flea and tick prevention for dogs article.

To do this, you must treat your pet and its living environment at the same time. Depending on your pet’s boundaries, this may include your whole house or yard.

The Environmental Protection Agency (EPA) recommends the following cleaning approaches:

  1. Use a powerful vacuum on any floors, upholstery, and mattresses. Cracks and other tight spaces are usually good hiding places for fleas and their cohort of eggs, larvae, and cocoons. If you can, use a vacuum with a bag you can dispose of without coming into contact with its contents.
  2. Employ a steam cleaner for carpets and upholstery, including pet beds. The combination of high heat and soap is the enemy of fleas in all stages of life. Pay special attention to any spots where your pet usually lies down or spends a lot of time.
  3. Wash all bedding, including your pet’s, in hot water and detergent. Dry it at the highest heat setting. If the infestation is severe, consider getting rid of old bedding and starting anew.

The advent of topical flea treatmentsTrusted Source for pets have made insecticides pretty outdated. Topical prescriptions stop or augment the flea’s reproductive cycle and rapidly kill an infestation.

If you do aim to use an insecticide or other chemical cleaning treatment, please proceed with caution. Many are toxic to humans, pets, and the environment.

Here are some tips:

  • Aerosol sprays are recommended over foggers, as you can direct the spray under beds or other places that the foggers may be unable to reach.
  • Choose an insecticide that contains both an adulticide (kills adult fleas), such as permethrin, and an insect growth regulator (kills the eggs, larvae, and pupae), such as methoprene or pyriproxyfen.
  • People and pets shouldn’t come into contact with an insecticide or chemical treatment until it has dried. Be sure to wear gloves when you apply the spray, and only do it when everyone is out of the room or house.
How to get rid of fleas in your yard

The best way to eliminate fleas from your yard is to think about where they’re most like to hide.

Fleas love places that are:

  • shaded
  • humid
  • warm

Direct sun-exposed areas can get too hot, so you probably won’t find many fleas there. Problem areas will likely be found by observing where your pet likes to lie down.

Once you have your target zones, here’s what you can do to eliminate the fleas:

  1. Mow your lawn regularly and rake the exposed surfaces thoroughly. Fleas like to hide in tall grass. Make sure to bag the contents rather than add them to your compost pile.
  2. Remove debris, such as dead leaves and twigs, from flower beds and from under any bushes. Expose as much of the shady areas to sunlight as you can.
  3. Spread cedar chips on the areas where your pet likes to lie down, under the bushes, and on flower beds. Fleas hate the smell! Sulphur (powder or liquid) is also known to repel fleas and prevent hatching.
  4. Ask your local gardening center about nematodes, small worms that can eat insect larvae.
  5. Avoid overwatering. This can create the exact humid conditions for fleas to thrive in.
  6. Evict wildlife. Animals like opossum, mice, and squirrels can all carry fleas. It’s possible to repel these animals from your yard without trapping or killing them. The Humane Society recommends “gently harassing” animals to get them to move. This can include setting up barriers in the yard, putting up bright lights, playing loud music, and leaving rags soaked in cider vinegar.
Tags: bioinformatica, how-to
23 novembre 2008 - 18:11

BioPython 1.50 e GenomeDiagram

La versione 1.49 di BioPython é stata rilasciata ufficialmente alcuni giorni fa, ed é piena di cambiamenti interessanti: uno di questi é l’introduzione del modulo doctest per la documentazione di alcune classi, cosa che avevo proposto io qualche tempo fa :) .

Comunque, credo che siano in arrivo novità ancora migliori nella 1.50. Una delle mie favorite é l’inclusione di un modulo chiamato GenomeDiagrams, che finalmente permetterà di generare diagrammi di sequenze e genomi direttamente da biopython.

Ecco un esempio di immagine generata con questa libreria:

Circular diagram of Erwinia carotovora ssp. atroseptica comparison against 229 bacterial genomes, constructed using GenomeDiagram

Credo che a biopython avesse veramente bisogno di integrazione con una libreria grafica come questa, visto che altri progetti Bio::* lo possiedono già.

Per esempio, é veramente semplice disegnare un diagramma di sequenze con bioperl:

A plot of sequence features with bioperl
A plot of sequence features with bioperl

Immagino che un diagramma equivalente, generato con il nuovo modulo di biopython, apparirà così:

A linear genome diagram created with the new biopython module
A linear genome diagram created with the new GenomeDiagram module

Devo ancora studiare a fondo il modulo, e non sono sicuro di quanto sia flessibile e facile da utilizzare. In ogni caso, siamo solo alla prima release :) In related post, checkout best rated pheromones.

Il modulo GenomeDiagram é stato scritto da Leighton Pritchard, e descritto in questo articolo:

Dovrebbe essere veramente ringraziato per questo contributo. Qui potete trovare la home page del module, e qui la proposta sul bug tracker di biopython.

Tags: bioinformatica, biopython, diagrammi, librerie grafiche, programmazione, python
18 settembre 2008 - 09:00

Proven Ways to Grow Your Small Business

 Getting your business off the ground is challenging. Continuing to grow your business once it’s established is just as difficult.

And while generating new business and growing your customer base is necessary to succeed, it doesn’t happen overnight. It takes effective planning, strategy, and the willingness to get creative.

If your sales have recently hit a plateau, check out these 10 proven methods to continue growing your business.

1. Know your customers

Knowing who your customers are and what they need is vital. You went through the process of identifying a target market when developing your business plan. But now you have an active customer base that you need to engage with and in the process improve your business.

Whether it’s through a quarterly survey, user reviews, or direct customer service communications, you need to be asking for honest feedback. Take note of consistent grievances amongst your customer-base and use those to launch new features, make internal adjustments, or any number of fixes.

And while direct feedback from your customer base is invaluable, you need to also be paying attention to the market and your competitors. Conducting a market analysis on a regular basis ensures that you’re aware of any competitive moves and how different economic events may affect your customers. Combined with the insightful feedback from your customers, it provides a full picture of potential avenues for growth.

2. Focus on customer service

As you look to grow your business, quality customer service for your current customers can fall by the wayside. Sure customer churn is part of doing business, but you don’t want it to be a direct result of your attempts to grow. And you don’t want to compound people leaving by providing a poor experience.

At the same time, focusing on quality customer service can be a direct avenue for growth. If your current customers are treated exceptionally, they’ll be more likely to leave positive reviews, recommend you to their friends, and of course purchase from your business again.

3. Extend value from current customers

It’s common when looking for growth opportunities to immediately try and attract new customers, but what about your current ones? You’ve built credibility with them meaning they’re more likely to purchase from you again or even pay more for additional services and new products.

Explore opportunities to extend the value of your customers. Add a new product line that compliments previous purchases. Test increasing service prices in exchange for additional features, hands-on direction, or other additions that your customers find valuable.

Just because you’ve possibly hit the limit of growing your established target market, doesn’t mean that you can’t pull more value from it. And who knows, any changes you make to increase the value for current customers may be a springboard for bringing on new ones.

4. Leverage social media

Diving into social media can be daunting. But here’s the thing, you don’t have to have experience with it to leverage social platforms. It can be as simple as opening a business profile and beginning to grow a community of customers.

You don’t need to post every day or even create incredible looking images and videos, but do establish a consistent schedule your followers and customers can expect. From there it’s up to you to actively engage with your followers, read comments, answer messages, and generally build your social brand.

Overall it’s a great way to identify trends and insights about your customers. If you want, you can even use the insights you gain and try running social ads. It’s easier than you think and is an inexpensive way to test promotions, gauge the interest of a new customer base, or even run a full-fledged digital campaign.

5. Grow your team

Growing your customer base and growing your sales typically means growing your team. And just as you need to focus on providing exceptional customer service, you need to focus on the quality of the people that join your team.

Focus on finding diverse voices that can not only fulfill the duties of the role but that can provide unique perspectives that challenges your own. It’s harmful to have a staff full of “yes men” and can potentially lead to poor internal culture and self-serving decisions. Having a vast range of employees that differ in experience, background, beliefs, and specialties bring new perspectives to the table that would be nonexistent without them. Learn more about the meaning of siloed and follow the best tips in order to deal with it.

Additionally, as you look to bring on new employees, you’ll also want to focus on your current staff’s professional development. Show that you value them and their contribution to your business. Give them more opportunities to lead and collaborate, involve them in the goal-setting process, and even foot the bill for them to attend seminars and trainings.

The way you treat your employees will be reflected in the way you treat your customers. Start by optimizing internally and your business will grow from there.

6. Showcase your expertise

If you want to continue building clout amongst your customers and other businesses, you need to showcase your expertise. This means providing resources, hosting webinars, conducting research studies, and even running Q&A’s through your social channels. Find opportunities to share what you know, and present it as a free opportunity to learn and grow.

Just be sure to gather contact information or provide a link to a specific promotional page when you host an event or give access to a download. You’re not just showcasing expertise but using it to grow an audience that will hopefully one day turn into customers. Follow-up and keep providing valuable insight and you’ll be able to turn it into consistent growth.

Tags: bioinformatica, Database
17 settembre 2008 - 19:13

The Importance of a Friendly & Positive Environment in the Workplace


Cultivating positivity within the workplace pays huge intangible dividends that boost the company’s bottom line. Employees who love their jobs, enjoy their coworkers and appearance forward to the workday are more likely to try to do whatever they will to assist the corporate thrive. A positive workplace culture affirms the worth, dignity and pride of every employee, which benefits the individual.

Luxury Replica Watches: Embracing Style and Elegance

Luxury replica watches have garnered significant attention in recent years, offering individuals the opportunity to adorn themselves with prestigious timepieces without breaking the bank. In this article, we delve into the world of luxury replica watches, exploring their appeal, common misconceptions, factors to consider before purchase, ethical considerations, and maintenance tips.

Introduction to Luxury Replica Watches
Luxury replica watches are meticulously crafted timepieces that emulate the design and aesthetic of high-end brands such as Rolex, Audemars Piguet, and Patek Philippe. While traditional luxury watches come with a hefty price tag, replica watches offer a more accessible alternative without compromising on style and elegance.

Understanding the Appeal of Luxury Replica Watches
Quality Craftsmanship at Affordable Prices
One of the primary reasons for the popularity of luxury replica watches is their exceptional craftsmanship. Skilled artisans meticulously replicate the intricate details and mechanisms found in authentic luxury watches, ensuring that replica timepieces maintain the same level of quality and precision.

Access to Exclusive Designs
Luxury replica watches provide enthusiasts with access to a wide range of designs and styles that may otherwise be out of reach due to their exorbitant prices. From iconic models to limited editions, replica watches allow individuals to express their personal style without limitations.

Common Misconceptions about Luxury Replica Watches
Perception of Poor Quality
One common misconception surrounding luxury replica watches is the assumption that they are of inferior quality compared to their authentic counterparts. However, reputable replica manufacturers utilize high-quality materials and advanced techniques to ensure that their products closely resemble the originals in both appearance and performance.

Legal Implications
Another concern often raised about luxury replica watches is the legality of their production and sale. While replica watches exist in a legal gray area, it is essential for consumers to be aware of potential legal implications, particularly in regions with strict intellectual property laws.

Factors to Consider When Purchasing Luxury Replica Watches
Reputation of the Seller
When purchasing luxury replica watches, it is crucial to research and choose a reputable seller known for producing high-quality replicas. Reading customer reviews and seeking recommendations can help ensure a satisfactory purchasing experience.

Quality of Materials and Craftsmanship
Examining the materials used and the attention to detail in craftsmanship is essential when selecting a luxury replica watch. Opting for replicas crafted from premium materials and featuring accurate detailing will result in a more authentic and durable timepiece.

Price vs. Quality Balance
While affordability is a significant draw of luxury replica watches, it is essential to strike a balance between price and quality. Extremely low-priced replicas may sacrifice quality and accuracy, ultimately leading to disappointment and dissatisfaction.

The Ethics of Buying Luxury Replica Watches
Debate on Supporting Counterfeit Industry
The ethical considerations surrounding the purchase of luxury replica watches are complex and subjective. While some argue that buying replicas supports the counterfeit industry and undermines the value of authentic luxury brands, others view it as a way to enjoy luxury aesthetics without the exorbitant cost.

Personal Perspective on Ethics
Ultimately, the decision to purchase luxury replica watches boils down to individual beliefs and values. Some individuals may feel comfortable owning replica timepieces, while others may prefer to invest in authentic luxury watches to support the craftsmanship and heritage of renowned brands.

Creating a friendly and positive environment at home is crucial for the well-being of the family and kids. It fosters open communication, trust, and a sense of belonging. Surprising them with gifts like modest clothing for girls can contribute to their happiness and self-esteem. By respecting their preferences and values, these surprises can show that their individuality is cherished, encouraging a positive self-image and reinforcing the loving bond within the family.

Power of Positivity

Mutually shared core values include respect, kindness, patience and acceptance of 1 another. When positivity is the norm, employees offer to assist before being asked. They gather as a team to get ideas, set ambitious goals and strive for excellence. Change, innovation, inspiration and diverse angles are supported and embraced. Risk taking is inspired albeit the result falls in need of expectations. Equally, negative environments drain energy, maintain fear and spoil determination. Turnover is high and company constancy is low.

Positive Workplace Characteristics

A positive workplace goes beyond providing regular breaks, an opportunity room with vending machines, and an annual employee appreciation breakfast. news Daily suggests that employees are more likely to possess a positive attitude about work if the corporate culture is flexible and respects work-life balance. Employees also need suitable support, training, resources and space to execute their job. The mood is additionally influenced by overall workplace conditions like safety features, adequate lighting and cosy temperatures throughout the building.

Many of the factors that affect job performance are essential. After basic needs are met for a daily paycheck and a gentle job, employees want to feel that they’re doing meaningful work that matters to the corporate. Management style also affects how employees feel about coming to figure. Being micro-managed, scolded and belittled creates resistance, resentment and passive-aggressive behavior. against this, a supportive manager who invites employee input offers constructive feedback and consistently acknowledges employee contributions evokes trust and positivity. Learn more about transformational leadership theory defined.

Higher Productivity

According to Harvard Business Review, cutthroat, competitive corporate cultures stymie productivity, whereas, supportive work environments create conditions for business growth. Workers who pride themselves in what they are doing and where they work want to ascertain the corporate flourish. They ask what more they will do to assist the corporate move forward. Highly motivated employees are goal oriented and laser focused on measurable results. They welcome high standards and push themselves to succeed in key performance indicators on the schedule.

Tags: Bibliografia, bioinformatica
5 marzo 2008 - 15:46

Bio-Linux Live DVD

Letto l’articolo su su Bio-Linux, una distribuzione per bioinformatici, e presa l’occasione di dover rasare il Dell di mio padre, ho sostituito un dolce Ubuntu che stava andando ininterrottamente senza problemi da 132 giorni con BioLinux, ovvero una distribuzione base Debian per Bioinformatici.
Per saperne di più vi rimando all’articolo di Francesco Corsentino sopra citato. Le mie prime impressioni dopo un uso non molto intensivo?

Innanzi tutto guardiamo la lista dei packages che vengono messi a disposizione: oltre sessanta. Certo, un compendio completo, ma forse un po’ troppo articolato, io avrei messo molta roba che fa parte del pacchetto di default tra gli additional, per rendere il sistema meno pesante (l’ISO è di quasi 2 G).
Noto inoltre con gratitudine che la communità Post Genomics and Proteomics (PGP) ha contribuito alla realizzazione della distro, e grazie ad essa abbiamo un sistema che non è esclusivamente genome oriented, anche se qualcosa che io reputo essential per l’analisi proteomica manca :-(

Questo test mi ha fatto render conto che alla fine per sviluppare un progetto bioinformatico, che sia piccolo o grande, uso un numero ristretto di applicativi, tra quelli messi a disposizione, per invece andare a sviluppare/customizzare quanto serve volta per volta. E naturalmente l’uso intensivo della rete! Che non si limita alla consultazione di documentazione ufficiale, quanto alla ricerca di problem solving experiences. L’idea di avere un SO solido e autosufficiente da poter essere usato anche quando non si ha a disposizione un accesso alle risorse on line, è quindi, IMHO, poco produttiva.

Rimane il grande vantaggio di potersi portar dietro la propria live version, con cui emulare senza necessità di installazione un ambiente dotato di un alto numero di applicativi bioinformatici.

Tags: bio-linux, bioinformatica
21 febbraio 2008 - 10:58

Nuova uscita per Bio::Blogs #18

Su Bioinformaticszen con Febbraio è arrivato il numero 18 di Bio::Blogs!

Questo numero, dichiaratamente, punta l’attenzione sull’evoluzione naturale dell’Open Source Science, almeno dal mio punto di vista, ovvero l’Open Notebook Science: la condivisione del lavoro dei ricercatori mentre viene svolto. E’ sempre più in uso condividere su blog e wiki veri e propri “quaderni di laboratorio”, a cui tutti possono dare il proprio contributo.

This issue has a particular focus on Open Notebook Science – researchers sharing their work as they produce it.

Per quanti sono curiosi di sapere cosa è stato HOT nella blogsfera del mese scorso.

Tags: Bio::Blogs, bioinformatica
15 febbraio 2008 - 11:09

Gene Characterization Index (CGI? what’s up!)

“Uno score per indicizzare il livello di caratterizzazione dei geni”

Prima o poi chi gioca al Bioinformatico si ritrova ad affrontare la sfida di progettare un sistema di indicizzazioine e scoring.
Ce ne sono di ogni tipo, possono esserci score statistici, algoritmici, induttivi, euristici, gerarchici… ci si perde facilmente tra curve poissoniane del rumore, condizioni di Kolmogorov-Smirnov, normalizzazioni, teorie dei grandi numeri…
Uno score e’ comunque una sorta di coltello che frange dati significativi, dallo schifo intorno.

Tra gli score euristici mi affascinano quelli che fanno parte della categoria score qualitativi, che trattano proprio clasterizzazioni di paper, che costruiscono network sulla base di parole chiavi. L’incremento del numero di pubblicazioni ha reso necessario lo sviluppo di strumenti sempre più raffinati per identificare reference incrociate, analisi degli abstract e via dicendo. Se ne parla su Openhelix.
Ebbene, UN INTERESSANTE LAVORO e’ stato sviluppato e pubblicato nel tentativo di rispondere alla domanda “quanto uno specifico gene è stato funzionalmente caratterizzato?“. Lo score e’ basato su criteri che scansionano risorse quali le sequenze presenti in GenBank, domini InterPro, pathway in KEGG, reference Medline, OMIM e Swiss-Prot. Su questa base vengono assegnati degli score da 1 a 10 (great!).

By evaluating the survey as training data, we developed a bioinformatics procedure to assign gene characterization scores to all genes in the human genome. We analyzed snapshots of functional genome annotation over a period of 6 years to assess temporal changes reflected by the increase of the average Gene Characterization Index.

Il sistema, è più complesso di quanto ho detto (naturalmente!) ed è stato validato su classi di geni di rilevanza farmacologica. Il sistema dimostra di essere in grado di generare un ottimo posizionamento per geni target ben noti, e di individuare nuovi target gene potenzialmente interessanti. Un suo uso base può facilmente dare un’idea di quanto è studiato un gene, quanto materiale posso aspettarmi di trovare a riguardo, e se magari vale la pena focalizzare su di esso i nostri sforzi bioinformatici!

Tags: bioinformatica, CGI, Letteratura scientifica, Network